首页|不同中医证型骨质疏松症患者肠道菌群的差异性分析

不同中医证型骨质疏松症患者肠道菌群的差异性分析

Differential Analysis of Gut Flora in Patients with Osteoporosis of Different Traditional Chinese Medicine Syndrome Types

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[目的]分析不同中医证型骨质疏松症患者肠道菌群特征及差异,为骨质疏松症诊治寻找新的治疗靶点.[方法]收集2023年2月～2023年11月于广州中医药大学第三附属医院骨质疏松科招募的57例骨质疏松症患者的临床资料及粪便样本,通过中医辨证分型分为脾肾阳虚组22例、肝肾阴虚组18例和肾虚血瘀组17例,采用16SrDNA测序技术分析3种中医证型患者肠道菌群结构和丰度的差异.[结果]Alpha(α)多样性分析结果显示,3组中医证型患者的肠道菌群物种丰富度、均匀度和多样性差异均无统计学意义(P＞0.05);Beta(β)多样性分析结果表明,3组中医证型患者的肠道菌群组间差异有统计学意义(P=0.011).在门水平上,3组中医证型患者占比最高的前3位均依次为拟杆菌门(Bacteroidota)、厚壁菌门(Firmicutes)、变形菌门(Proteobacteria).在属水平上,肝肾阴虚组、肾虚血瘀组中拟杆菌属(Bacteroides)、大肠埃氏菌属-志贺氏菌属(Escherichia-Shigella)、粪杆菌属(Faecalibacterium)在相对丰度上具有优势,而脾肾阳虚组则是其他菌的占比较大.[结论]不同中医证型患者间的肠道菌群构成存在差异,在门及属水平上均有所不同,提示肠道菌群可能对中医防治骨质疏松起到靶点作用.
Objective To analyze the characteristics and differences of gut flora in patients with osteoporosis of different traditional Chinese medicine(TCM)syndrome types,thus to find out new therapeutic targets for the treatment of osteoporosis.Methods The clinical data and fecal samples of 57 patients with osteoporosis recruited in the Department of Osteoporosis,the Third Affiliated Hospital of Guangzhou University of Chinese Medicine from February 2023 to November 2023 were collected.The patients were differentiated as the TCM syndrome types of spleen and kidney yang deficiency group(22 cases),liver and kidney yin deficiency group(18 cases)and kidney deficiency and blood stasis group(7 cases).The differences in the structure and abundance of gut flora in patients with the above three TCM syndrome types were analyzed by16S rDNA sequencing technique.Results The results of Alpha(α)diversity showed that there were no significant differences in species richness,evenness and diversity of gut flora among the three groups(P＞0.05).Beta(β)diversity analysis showed that there were significant differences in gut flora among the three groups(P=0.011).At the phylum level,Bacteroidota,Firmicutes and Proteobacteria were the top three kinds of gut flora with the highest proportion in the three groups.At the genus level,the relative abundance of Bacteroides,Escherichia-Shigella,and Faecalibacterium was higher in the group of liver and kidney yin deficiency and in the group of kidney deficiency and blood stasis,while the group of spleen and kidney yang deficiency has a large proportion of other bacteria.Conclusion There exist differences in the composition of gut flora among patients with different TCM syndrome types,and the differences are shown at the level of phylum and genus.The results indicate that gut flora may be the targets in the TCM prevention and treatment of osteoporosis.

osteoporosisgut floratraditional Chinese medicine(TCM)syndrome typesspleen and kidney yang deficiencyliver and kidney yin deficiencykidney deficiency and blood stasismicrobiota

周志城、田瀚、庄旭锐、梁祖建

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广州中医药大学,广东广州 510405

广州中医药大学第三附属医院,广东广州 510240

骨质疏松症 肠道菌群 中医证型 脾肾阳虚 肝肾阴虚 肾虚血瘀 微生物

广东省自然科学基金项目

2021A1515011469

2024

10.13359/j.cnki.gzxbtcm.2024.09.010

广州中医药大学学报
广州中医药大学

广州中医药大学学报

CSTPCD
影响因子：1.055
ISSN：1007-3213
年,卷(期)：2024.41(9)