Therapeutic Effect of Huayu Shapei Decoction Combined with Methotrexate in the Treatment of Ectopic Pregnancy and Its Effect on TGF-β/Smad2/3 Protein Level and microRNA-30d-5p Expression
Objective To investigate the effect of Huayu Shapei Decoction(mainly composed of Salviae Miltiorrhizae Radix et Rhizoma,Paeoniae Radix Rubra,Persicae Semen,Scolopendra,Arnebiae Radix,Trichosanthis Radix,and Notoginseng Radix et Rhizoma)combined with Methotrexate in the treatment of ectopic pregnancy,and to observe its effect on TGF-β/Smad2/3 protein level and microRNA-30d-5p expression in the patients.Methods A total of 104 patients with ectopic pregnancy were randomly divided into an observation group and a control group,52 cases in each group.The control group was treated with Methotrexate combined with Mifepristone,and the observation group was treated with Huayu Shapei Decoction on the basis of treatment for the control group.The patients were treated for three continuous days and then were followed up for one month.The success rate of conservative treatment,the time for blood β-human chorionic gonadotropin(β-HCG)level returning to normal(β-HCG recovery time),the time for the absorption of ectopic pregnancy mass,the complete patency rate and non-patency rate of fallopian tube after treatment between the two groups were compared.The changes of serum TGF-β/Smad2/3 protein level and microRNA-30d-5p relative expression before and after treatment in the patients with successful conservative treatment in the two groups were observed.Results(1)The success rate of conservative treatment in the observation group was 94.23％(49/52),which was significantly higher than 71.15％(37/52)in the control group,and the difference was statistically significant(P＜0.05).(2)Compared with the control group,the blood β-HCG recovery time and mass absorption time in the observation group were significantly shortened,and the differences were statistically significant(P＜0.01).(3)The complete patency rate of fallopian tube after treatment in the observation group was 55.77％(29/52),which was significantly higher than that in the control group(21.15％,1 1/52);the non-patency rate in the observation group was 19.23％(10/52)and that in the control group was 59.62％(31/52),and the difference was statistically significant(P＜0.05 or P＜0.01).(4)Compared with those before treatment,the levels of TGF-β/Smad2/3 protein and the relative expression level of microRNA-30d-5p in the patients with successful conservative treatment in the two groups one month after the end of treatment were significantly decreased(P＜0.05 or P＜0.01);the inter-group comparison showed that compared with the control group,the TGF-β/Smad2/3 protein level and the relative expression level of microRNA-30d-5p in the observation group were significantly decreased one month after the end of treatment,and the differences were statistically significant(P＜0.01).(5)The adverse reactions of the two groups were mainly manifested as gastrointestinal discomforts.The incidence of adverse reactions in the observation group was 25.00％(13/52),which was significantly lower than that in the control group(46.15％,24/52).The difference between the two groups was statistically significant(P＜0.05).Conclusion For the treatment of ectopic pregnancy,Huayu Shapei Decoction combined with Methotrexate is effective on improving the success rate of conservative medicinal treatment,promoting the absorption of mass and tubal patency,decreasing the level of TGF-β/Smad2/3 protein and the relative expression of microRNA-30d-5p.The safety of drug therapy is high.
万方数据
维普
