Therapeutic Effect of Leonurine on Hepatitis B Rats by Regulating the RhoA/ROCK Signaling Pathway
Objective To observe the therapeutic effect and mechanism of Leonurine on hepatitis B rats.Methods Sixty rats were randomly divided into six groups,i.e.,the normal group,the model group,the Leonurine low-,medium-and high-dose groups,and the Leonurine high-dose+lysophosphatidic acid(LPA)group.Hepatitis B model was constructed for rats in all groups except for the normal group.After successful modeling,each group was given corresponding invention.At the end of administration,the levels of serum hepatitis B e antigen(HBeAg),hepatitis B surface antigen(HBsAg),and inflammatory factors such as interleukin(IL)-10,IL-6 and tumor necrosis factor α(TNF-α)were detected by enzyme-linked immunosorbent assay(ELISA),and levels of serum alanine aminotransferase(ALT)and aspartate transaminase(AST)were detected by automatic analyzer.Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect HBV DNA level in liver tissues,hematoxylin-eosin(HE)staining was used to observe the pathological changes in liver tissues,immunohistochemistry was used to detect the expression of hepatitis B core antigen(HBcAg)in liver tissues,and Western Blot was used to detect the expression of Ras homolog gene family member A(RhoA),Rho-associated coiled-coil containing kinases(ROCK)1 and ROCK2.Results Compared with the normal group,necrosis in the center of hepatic lobules,edema and degeneration of hepatocytes in rats was showed in the model group,and the levels of HBsAg,HBeAg and HBV DNA,HBcAg-positive cell counts,the levels of ALT and AST,the contents of IL-6,TNF-α and IL-10,and the expression levels of RhoA,ROCK1,ROCK2 were significantly increased(all P＜0.05).Compared with the model group,liver damage degree was decreased and cellular degeneration was reduced in rats in the Leonurine low-,medium-and high-dose groups,the levels of HBsAg,HBeAg and HBV DNA,HBcAg-positive cell counts,the levels of ALT and AST,the contents of IL-6 and TNF-α,and the expression levels of RhoA,ROCK1 and ROCK2 were significantly decreased,and the IL-10 level were significantly increased,the differences being statistically significant(P＜0.05).Compared with the Leonurine high-dose group,the degree of liver damage was increased in the Leonurine high-dose+LPA group,the levels of HBsAg,HBeAg and HBV DNA,HBcAg-positive cell counts,the levels ALT and AST,contents of IL-6 and TNF-α,and the expression levels of RhoA,ROCK1 and ROCK2 were significantly increased,and IL-10 level was significantly decreased,the differences being statistically significant(P＜0.05).Conclusion Leonurine can relieve liver injury,improve liver function and reduce inflammatory reaction in hepatitis B rats,and its mechanism is related to the inhibition of RhoA/ROCK pathway.
Leonurinehepatitis Bliver functioninflammatory reactionRhoA/ROCK pathwayrats
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