Effects of Huazhuo Jiedu Tiaogan Prescription on Lipid Peroxidation,Macrophage Polarization and CCL22/CCR4 in Rats with Hepatic Fibrosis
Objective To observe the therapeutic efficacy and mechanism of Huazhuo Jiedu Tiaogan Prescription in rats with hepatic fibrosis(HF).Methods Sixty rats were randomly divided into normal group,model group,Chinese medicine low-,medium-and high-dose groups,and western medicine group,10 rats in each group.Except for the normal group,the rats in all groups were established HF model.After successful modeling,the rats in Chinese medicine low-,medium-and high-dose groups were given gastric administration of water decoction of Huazhuo Jiedu Tiaogan Prescription,and the rats in western medicine group were given gastric administration of colchicine saline solution.After 28-day administration,the serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and alkaline phosphatase(ALP)were measured by an automatic biochemical analyzer,the pathological morphology of the liver tissues was observed by hematoxylin-eosin(HE)staining method,and collagen deposition of the liver tissues was detected by Masson staining,and the levels of lipid peroxidation indicators in liver tissues such as superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)and catalase(CAT)were detected by enzyme-linked immunosorbent assay(ELISA),and the positive expression of M2-type cluster of differentiation 163(CD163),M1-type cluster of differentiation 68(CD68),which are macrophage surface markers in liver tissues,were detected by immunohistochemical assay,Western Blot method was used to detect the protein expression of C-C motif chemokine 22(CCL22)and CC chemokine receptor 4(CCR4)in liver tissues.Results Compared with the normal group,the levels of liver function indicators including ALT,AST and ALP in rat serum were elevated in the model group,the levels of SOD,GSH-Px and CAT in liver tissues were decreased and level of MDA was decreased,the level of CD163 positive expression was decreased and level of CD68 positive expression was increased in liver tissues,and the protein expression levels of CCL22 and CCR4 were elevated in liver tissues,the differences being all statistically significant(P＜0.05).Compared with the model group,the levels of serum ALT,AST and ALP in the Chinese medicine low-,medium-and high-dose groups as well as the western medicine group were decreased,the levels of liver tissue SOD,GSH-Px and CAT were increased,and MDA level was decreased,liver tissue CD163 positive expression level was increased and CD68 positive expression level was decreased,and the protein expression levels of CCL22 and CCR4 in liver tissues were decreased,differences being statistically significant(P＜0.05).The levels of the above indicators(except for ALT and ALP)in the high-dose of Chinese medicine group showed that the differences was not statistically significant compared with those in the western medicine group(P＞0.05).Conclusion The anti-fibrosis effect of Huazhuo Jiedu Tiaogan Prescription is related to the inhibition of lipid peroxidation,accelerating the transformation of M1-type macrophages to M2-type,and the inhibition of CCL22/CCR4 expression.
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