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番茄青枯病拮抗菌的筛选及防治效果

Screening and control effects of antagonistic bacteria against tomato bacteria wilt

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为了筛选出具有抑制青枯雷尔氏菌作用的拮抗菌,采用形态指标、生理生化特征以及16S rRNA序列分析等方法对菌株进行了研究,并且通过阴性对照(CK)、番茄青枯病菌阳性胁迫处理(RB)和拮抗菌K+番茄青枯病菌处理(KRB)3个盆栽番茄土壤样品进行了防卫反应基因的表达研究.结果表明,拮抗菌K5菌落呈乳白色且表面光滑,经革兰氏染色为阳性,初步确定为芽孢杆菌,通过分子鉴定后确定为贝莱斯芽孢杆菌;番茄青枯病的盆栽防治效果达到23.20%,K5菌株在接种48 h后能诱导番茄根部抗病基因表达,CTR、ETR基因的表达量提高,表明K5通过植物抗病相关基因的表达,能诱导植物产生抗病性,并能有效地抑制青枯病菌的繁殖,从而达到防治青枯病的作用.
In the report,in order to obtain the antagonistic bacteria with inhibitory effects against tomato bacteria wilt,the morphological indicators,physiological and biochemical characteristics,and 16S rRNA sequence anal-ysis were used to study the strains,and three potted tomato soil samples,negative control(CK),positive stress treatment(RB)and antagonistic bacterium K+tomato cyanobacteria treatment(KRB)were used to analyze the expression of defense response genes.The results indicated that the antagonistic bacterium K5 colonies were creamy white with smooth surface,Gram-positive,Bacillus,and which were identified as Bacillus velezensis by molecular identification.The inhibitory effects of Pot control of tomato cyanobacteria reached 23.20%,y the K5 strain was able to induce the expression of disease resistance genes in tomato roots 48 h after inoculation,and the expression of CTR and ETR genes were increased,which suggested that K5 induced plant disease resistance through the expression of plant disease-resistance related genes and thus disease resistance,which can effective-ly inhibit the propagation of tomato bacteria wilt and thus achieve the effect of tomato bacteria wilt control.

tomato bacteria wiltantagonistic strainsscreeningidentification

王位、薛鸣、雷婷越、安馨媛、范鹤龄、张荣萍

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海南大学热带作物学院,海南海口 570228

海南大学植物保护学院,海南海口 570228

番茄青枯病 拮抗菌 筛选 鉴定

海南省自然科学基金

323RC419

2024

海南大学学报(自然科学版)
海南大学

海南大学学报(自然科学版)

影响因子:0.524
ISSN:1004-1729
年,卷(期):2024.42(1)
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