克氏双锯鱼(Amphiprion clarkii)CRH基因的克隆及表达分析
Cloning and tissue expression profiling of corticotropin-releasing hormone(CRH)in yellowtail clownfish,Amphiprion clarkii
郑德才 1张娴 1黄泽翔 1张浩 1王茜1
作者信息
- 1. 海南大学海洋学院,海南海口 570228
- 折叠
摘要
为探讨克氏双锯鱼CRH基因的分子表达模式及其在不同社会地位个体下丘脑中的表达差异,本研究克隆得到CRH基因cDNA全长序列1 137 bp,编码168个氨基酸.系统发育树显示,克氏双锯鱼CRH基因与欧洲鲈鱼(Dicentrarchus labrax)、黄斑蓝子鱼(Siganus canaliculatus)亲缘关系最近.运用 real-time qPCR技术,检测了社会地位未形成时克氏双锯鱼CRH基因的组织分布,结果显示,CRH基因在下丘脑中显著高表达;接着对不同社会地位克氏双锯鱼CRH基因在下丘脑中的表达差异进行检测,结果显示,功能雌性CRH基因的表达显著高于功能雄性及非功能雄性个体,提示CRH基因可能参与不同社会地位克氏双锯鱼性腺发育的调节.
Abstract
In the report,in order to explore the molecular expression pattern of the CRH gene and its differential expression in individuals with different social status,the full-length cDNA sequence of 1137 bp was cloned,which encode 168 amino acids.The phylogenetic tree analysis results showed that the CRH gene of the yellow-tail clownfish was most closely related to that of the European perch(Dicentrarchus labrax)and the yellow-spot-ted bluegill(Siganus canaliculatus).Real-time qPCR was used to detect the tissue distribution of the CRH gene in yellowtail clownfish,and the results showed that the CRH gene was significantly more highly expressed in the hypothalamus;the different expression of the CRH gene in the hypothalamus was then examined in different so-cial status of yellowtail clown-fish,the results showed the expression of the CRH gene was significantly higher in functional females than that in functional males and non-functional males,which suggested that the CRH gene may be involved in the regulation of gonadal development in different social status of yellowtail clownfish.
关键词
克氏双锯鱼/促肾上腺激素释放激素/社会地位Key words
Amphiprion clarkia/corticotropin-releasing hormone/social status引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据