Isolation and Identification of Flavonoids from Hedychium coccineum
The air-dried and crushed rhizomes of Hedychium coccineum were extracted with MeOH,and the extract was partitioned with EtOAc-H2O.The EtOAc extract was isolated by various separation techniques(Silica gel,Sephadex LH-20,RP-18 column chromatography and HPLC).By means of spectroscopic analysis,the isolated compounds were identi-fied as 5-hydroxy-3,7,4'-trimethoxyflavone(1),3,5-dihydroxy-7,4'-dimethoxyflavone(2),kaempferol-4'-O-methylether(3),ermanin(4),dihydrokaempferide(5)and aromadendrin-7,4'-dimethyl ether(6).All the compounds were isolated from H.coccineum for the first time.