基于多壁碳-Au/rGO纳米复合材料的生物传感器的制备及其在有机磷农药检测中的应用
Preparation of the Biosensor Based on Multi-wall Carbon-Au/rGO Nanocomposites and Its Application in the Detection of Organophosphorus Pesticides
汪明畅 1张钰桐 1苗莎菻 1王瑜 1张晓萍1
作者信息
- 1. 海南师范大学 化学与化工学院,海口市功能材料与光电化学重点实验室,海南 海口 571158
- 折叠
摘要
本文研究了多壁碳纳米管和金复合还原石墨烯(Au/rGO)修饰电极的电化学性能,通过吸附法将乙酰胆碱酯酶固定到修饰电极表面制成生物传感器,并将其用于有机磷农药的检测.结果表明,在1.0×10-6～1.0×10-11 mol/L范围内辛硫磷对酶的抑制率与辛硫磷浓度的负对数呈线性关系,线性方程为I=-0.061 0 lgc+1.222 01,相关系数与检出限分别为0.985和8.185×10-7 mol/L.采用循环伏安法(CV)和差分脉冲伏安法(DPV)实现了对辛硫磷样品的分析检测,回收率为91.0%～110.0%.该传感器可用于有机磷农药残留检测.
Abstract
In this paper,the electrochemical properties of multi-walled carbon nanotubes(MWNTS)and gold composite re-duced graphene(Au/rGO)modified electrodes were investigated,and acetylcholinesterase was immobilized on the surface of modified electrodes by adsorption method to make biosensor,which was used for the detection of organophosphorus pesti-cides.The results showed that there was a linear relationship between the inhibition rate of phoxim and negative logarithm of phoxim concentration in the range of 1.0×10-6～1.0×10-11 mol/L,the linear equation was I=-0.611 0 lgc+1.222 01,the correlation coefficient and detection limit were 0.985 and 8.185×10-7 mol/L.Cyclic voltammetry(CV)and differential pulse voltammetry(DPV)were used to analyze and detect phoxim samples.The recoveries were 91.0%-110.0%.The sensor can be used to detect organophosphorus pesticide residues.
关键词
多壁碳/Au/rGO/乙酰胆碱酯酶/传感器/有机磷农药Key words
multi-walled carbon/Au/rGO/acetylcholinesterase/sensor/organic phosphorus pesticide引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据