Preparation of the Biosensor Based on Multi-wall Carbon-Au/rGO Nanocomposites and Its Application in the Detection of Organophosphorus Pesticides
In this paper,the electrochemical properties of multi-walled carbon nanotubes(MWNTS)and gold composite re-duced graphene(Au/rGO)modified electrodes were investigated,and acetylcholinesterase was immobilized on the surface of modified electrodes by adsorption method to make biosensor,which was used for the detection of organophosphorus pesti-cides.The results showed that there was a linear relationship between the inhibition rate of phoxim and negative logarithm of phoxim concentration in the range of 1.0×10-6～1.0×10-11 mol/L,the linear equation was I=-0.611 0 lgc+1.222 01,the correlation coefficient and detection limit were 0.985 and 8.185×10-7 mol/L.Cyclic voltammetry(CV)and differential pulse voltammetry(DPV)were used to analyze and detect phoxim samples.The recoveries were 91.0%-110.0%.The sensor can be used to detect organophosphorus pesticide residues.
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