Detection of Specific Sequences of Escherichia coli by Electrochemical Gene Sensors Based on Graphyne-Au Composite Modified Electrode
In this paper,we prepared a graphyne-Au nanocomposite modified carbon ionicliquid electrode(Au/GDY/CILE)as the working electrode using drop-coating and constant potential deposition methods.Cyclic voltammetry(CV)experiments showed that the Au-GDY composite could effectively improve the electrochemical properties of the modified electrode interface,and the modified probe ssDNA was successfully immobilized onto the surface of the working electrode based on gold-sulfur bond self-as-sembly effect,which was used to construct a gene sensor for the detection of specific sequences of Escherichia coli(E.coli).By us-ing methylene blue(MB)as an electrochemical indicator,the sensor demonstrated a broad detection range(1.0×10-15 mol/L to 1.0×10-7 mol/L)and an impressively low detection limit(3.17×10-16 mol/L)and high sensitivity[2.91 µA/(M·cm2)]for the detection of characteristic gene sequence of E.coli through the application of differential pulse voltammetry(DPV).
Au/GDYnanocompositeelectrochemical gene sensorscharacteristic gene sequence of E.colimethylene blue
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