摘要
本文研究鲜品百合鳞茎正丁醇部位的化学成分.采用硅胶柱层析、大孔树脂、葡聚糖凝胶和半制备高效液相色谱仪等分离方法对鲜品百合鳞茎95%乙醇提取物的正丁醇部位进行分离纯化,并结合理化性质和NMR等波谱学技术对化合物进行结构鉴定.从鲜品百合鳞茎正丁醇部位分离得到16个化合物,分别鉴定为:3'-O-feruloyl-6-O-(4-O-glucopyranosyl)feruloylsucrose(1)、3',6-di-O-feruloylsucrose(2)、王百合苷A(3)、王百合苷B(4)、王百合苷C(5)、王百合苷E(6)、王百合苷F(7)、2,2-[bis-4-(2,3-dihydroxypropoxy)phenyl]propane(8)、黄芩苷(9)、芦丁(10)、齐墩果酸(11)、β-谷甾醇(12)、儿茶素(13)、表儿茶素(14)、山柰酚(15)和槲皮素(16).其中,化合物1、2、4、6、7和9为首次从百合属中分离得到.
Abstract
To study the chemical constituents of n-butanol extract from the fresh bulbs of Lilium brownii,the n-butanol portion of 95%ethanol extract from the fresh bulbs of Lilium brownii was separated and purified by column chromatography such as silica gel column chromatography,macroporous resin,sephadex LH-20 and semi-preparative HPLC,and the structures of the isolated compounds were identified by combining physical and chemical properties and NMR spectroscopy.Sixteen compounds were iso-lated and identified as 3'-O-feruloyl-6-O-(4-O-glucopyranosyl)feruloylsucrose(1),3',6-di-O-feruloylsucrose(2),regaloside A(3),regaloside B(4),regaloside C(5),regaloside E(6),regaloside F(7),2,2-[bis-4-(2,3-dihydroxypropoxy)phenyl]propane(8),baicalin(9),rutin(10),olonolic acid(11),β-sitosterol(12),catechin(13),epicatechin(14),kaempferol(15),and quercetin(16)respectively.Among them,compounds 1,2,4,6,7 and 9 were first isolated from the genus of Lilium.
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