摘要
本研究对一株海洋来源细菌(Serratia ureilytica)的次级代谢产物进行系统分离.采用多种柱色谱分离方法从该菌中分离得到16个单体化合物,运用 NMR技术鉴定所得化合物分别为:环(色氨酸-丝氨酸)(1)、环(甘氨酸-色氨酸)(2)、环(苯丙氨酸-谷氨酰胺)(3)、环(亮氨酸-谷氨酰胺)(4)、环(L-酪氨酸-4-羟基-D-脯氨酸)(5)、环(L-苯丙氨酸-4-羟基-D-脯氨酸)(6)、环(L-苯丙氨酸-4-羟基-L-脯氨酸)(7)、环(L-4-羟基-脯氨酸-L-亮氨酸)(8)、环(脯氨酸-酪氨酸)(9)、环(D-脯氨酸-L-酪氨酸)(10)、环(S-脯氨酸-S-亮氨酸)(11)、环(L-脯氨酸-L-缬氨酸)(12)、环(酪氨酸-甘氨酸)(13)、环(R-苯丙氨酸-S-丝氨酸)(14)、环(L-苯丙氨酸-甘氨酸)(15)和环(甘氨酸-亮氨酸)(16).化合物1～16均为首次从解脲沙雷菌中分离得到.抗菌活性显示,粗提物对青枯菌有显著的拮抗作用,而单体化合物对青枯菌以及5株人体致病菌均无明显活性.
Abstract
The secondary metabolites of marine bacteria(Serratia ureilytica)were studied systematically,and sixteen com-pounds were isolated by various chromatographic methods and the structures of these compounds were identified by 1D and 2D NMR.The compounds were identified to be cyclo-(L-Trp-L-Ser)(1),cyclo-(Gly-L-Trp)(2),cyclo-(Phe-Gln)(3),cyc-lo-(Leu-Gln)(4),cyclo-(L-tyrosyl-cis-4-hydroxy-D-proline)(5),cyclo-(L-phenylalanine-D-4-hydroxyproline)(6),cyc-lo-(L-4-OH-Pro-L-Phe)(7),cyclo-(L-4-Hyp-L-Leu)(8),cyclo-(L-Pro-L-Tyr)(9),cylco-(D-Pro-L-Tyr)(10),cyclo-(S-Pro-S-Leu)(11),cyclo-(L-Pro-L-Val)(12),cyclo-(Tyr-Gly)(13),cyclo-(R-Phe-S-Ser)(14),cyclo-(L-Phe-Gly)(15),and cyclo-(Gly-Leu)(16)respectively.All the compounds were isolated from the S.ureilytica for the first time.The antibac-terial activity showed that the crude extract had significant antagonistic effect on Ralstonia solanacearum,while,no com-pound showed antibacterial activity against Ralstonia solanacearum and 5 strains of human pathogenic bacteria.
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