Protective effect of camellia oil on H2O2-induced oxidative stress injury in H9C2 cardiomyocytes of rats
Objective:To investigate the protective effect of camellia oil on H2O2-induced oxidative stress injury in H9C2 cardiomyocytes.Methods:The survival rate of H9C2 cardiomyocytes treated with different concentrations of H2O2 was detected by CCK8 method.Normal cultured cells were treated with 200 μmol/L H2O2 for 24 h,and the oxidative stress injury model was established as the model group.Cells were pretreated with 1%,0.1%,0.01%concentration of camellia oil for 24 h,and H2O2 was added for 24 h as the experimental group.The changes of cell senescence,mitochondrial membrane potential,proliferation,apoptosis and migration in each group were observed by β-galactosidase senescence staining,mitochondrial membrane potential staining,EdU cell proliferation staining and scratch staining respectively.The activity of superoxide dismutase(SOD),lactate de-hydrogenase(LDH)and malondialdehyde(MDA)in each group were detected by the kit.Results:The viability H9C2 cardiomyo-cytes treated with different concentrations of H2O2 inhibited and positively correlated with H2O2 concentration(P<0.01).Com-pared with the blank control group,the positive rate of cell senescence,MDA content and LDH activity in the H2O2 model group were increased(P<0.01).Mitochondrial membrane potential,cell proliferation rate,mobility and SOD activity were decreased(P<0.01).Compared with the H2O2 model group,the senescence positive rate(P<0.01 or P<0.05),MDA content and LDH ac-tivity were decreased in the experimental group(P<0.01 or P<0.05).Mitochondrial membrane potential increased,cell prolifera-tion rate and mobility were increased(P<0.01 or P<0.05).Conclusion:Camellia oil can significantly inhibit the oxidative stress injury of H9c2 cells and play a protective role in cardiomyocytes.
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