Effect of Chuanzhifang component(ZGC)on macrophage inflammatory injury based on whole gene expression profile
Objective:To analyze the effect of Chuanzhi Fang(ZGC)on the whole genome expression profile of RAW264.7 cells activated by lipopolysaccharide(LPS),and to explore the possible mechanism of action and core target of this formula on macrophage inflammatory injury at the overall level.Methods:A model of LPS-induced inflammation in RAW264.7 cells was con-structed,and the effect of ZGC intervention on the genome-wide expression of inflammatory macrophages was examined by gene microarray technology,GO/KEGG enrichment analysis was performed for significantly differentially expressed genes among each group.Results:The results of genome-wide expression profiling microarray analysis showed that the ZGC intervention group up-regulated the expression of 5 genes including C4bp and inhibited the expression of 22 genes including Mgat3,Psma6,and Siglecg relative to the LPS model group.KEGG signaling pathway analysis results showed that ZGC mainly acted through cytokine recep-tor interaction and the C-type lectin receptor signaling pathway.Conclusion:ZGC can interfere with the abnormal expression of 27 genes in inflammatory macrophages,and the related genes may exert corresponding anti-inflammatory effects by affecting cytokine receptor interactions,C-type lectin receptor signaling pathway,and TLR4/NF-κB signaling pathway.
Chinese herbal medicine component Chuanzhifang(ZGC)RAW264.7 cellCytokineGene expression profil-ing
万方数据
维普
