Differential expression of miRNA in THP-1-derived foam cell model induced by drug-containing serum of Yimaijiangzhi decoction
Objective:To investigate the differential expression of miRNA and related biological functions and signaling path-ways after the intervention of the THP-1-derived foam cell model with the drug-containing serum of Yimaijiangzhi decoction.Methods:The experiment was divided into macrophage group,foam cell model group and drug-containing serum of Yimaijiangzhi-group.THP-1 cells were induced into macrophages by Fopol ester,and the induced differentiated macrophages were given ox-LDL to establish foam cell model,and rat serum Yimaijiangzhi decoction was used to intervene the foam cells.Total RNA was extracted from cells in each group for miRNA sequencing,differential expression of miRNA was screened,and relevant target genes were predicted for GO analysis and KEGG analysis,protein interaction network and miRNA-target gene interaction net-work were established,and RT-qPCR was used to verify the possible signaling pathways for improving atherosclerosis.Result:The difference miRNA between blank group and model group was hsa-miR-302c-3p,hsa-miR-302d-3p,hsa-miR-30d-3p,hsa-miR-3189-3p,hsa-miR-374b-5p,hsa-miR-423-5p,hsa-miR-423-5P,and hsa-miR-4781-3p as well as hsa-miR-663a;The miR-NAs of model group and drug-containing serum group of Yimaijiangzhi were hsa-miR-3150a-3p,hsa-miR-7704,hsa-miR-877-3p,hsa-miR-150-5p,hsa-miR-423-5p,hsa-miR-374c-3p,hsa-mir-374c-3p,and hsa-miR-374b-5p;The difference of miRNAs predic-tion target genes between model group and drug-containing serum group of Yimaijiangzhi showed that the miRNA prediction target genes were mainly enriched in MAPK signaling pathway,ErbB signaling pathway,Hippo signaling pathway,Wnt signaling path-way and other signaling pathways.SCN1A,PRKACA,MECP2,EIF4E,SRSF1,MBNL1,PRKCA,and PPARGC1A may be the potential key targets for the effect of the drug-containing serum of Yimaijiangzhi decoction on THP-1-derived foam cells.Con-clusion:hsa-miR-374c-3p,hsa-miR-423-5p,and hsa-miR-374b-5p are important miRNAs that the drug-containing serum of Yimaijiangzhi Decoction acts on foam cells.The significantly differentially expressed miRNAs and significantly enriched related signaling pathways may provide new ideas for the diagnosis and treatment of atherosclerosis.
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