Research on hsa-miR-155-3p and hsa-miR-155-5p as biomarkers for systemic sclerosis and their role in regulating biological behavior
Objective:This study was to investigate the role of hsa-miR-155-3p and hsa-miR-155-5p as biomarkers and regula-tors of biological behavior in systemic sclerosis(SSc).Methods:A total of 10 SSc patients and 10 healthy controls were selected for the study.The expression levels of hsa-miR-155-3p and hsa-miR-155-5p in peripheral blood mononuclear cells of SSc patients and healthy controls were measured using RT-qPCR.The diagnostic value of these miRNAs was explored using Receiver Operat-ing Characteristic curve analysis.Pearson or Spearman correlation analysis was performed to assess the correlation between miR-NAs and clinical indicators in SSc patients.Potential target genes of hsa-miR-155-3p and hsa-miR-155-5p were predicted using miRDB,Targetscan,and miRDIP databases.GO functional annotation,KEGG pathway enrichment analysis,protein-protein in-teraction network construction,and selection of central genes were conducted.Results:The expression levels of hsa-miR-155-3p and hsa-miR-155-5p were significantly higher in PBMCs of SSc patients compared to healthy controls(P<0.001).The ROC curve analysis showed that hsa-miR-155-3p and hsa-miR-155-5p had a high diagnostic value for SSc(AUC=1,P<0.001).Corre-lation analysis revealed that hsa-miR-155-3p,hsa-miR-155-5p,and clinical indicators such as high-resolution CT,neutrophil per-centage,lymphocyte percentage,and albumin to globulin ratio were correlated(P<0.05).The signaling pathways enriched with target genes of hsa-miR-155-3p and hsa-miR-155-5p were closely associated with the occurrence and development of SSc fibrosis,immunity,and inflammation.Conclusions:Hsa-miR-155-3p and hsa-miR-155-5p may be involved in regulating the occurrence and development of SSc fibrosis,immunity,and inflammation.They have the potential to serve as biomarkers for clinical diagno-sis and treatment of SSc.
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