Effects of esomeprazole combined with EGFR inhibitor AG1478 on proliferation,apoptosis and autophagy of gastric cancer cells
Objective:To investigate the effects and molecular mechanisms of esomeprazole combined with epidermal growth factor receptor(EGFR)inhibitor AG1478 on proliferation,migration,apoptosis and autophagy of gastric cancer cells.Methods:The experiment was divided into blank control group,esomeprazole group,AG1478 group and esomeprazole combined with AG1478 group.The viability,proliferation,migration,apoptosis and autophagy changes of human gastric cancer AGS and SGC-7901 cells in each group were detected by CCK-8 method,EdU proliferation assay,Transwell,flow cytometry and cell im-munofluorescence respectively.Western blotting was performed to detect the expression of EGFR,c-MYC,P53,cleaved-PARP,LC3B,PI3K,AKT,P-AKT proteins.Results:AG1478 inhibited the viability and EGFR expression of gastric cancer cells in a concentration-dependent manner.Esomeprazole and AG1478 significantly reduced gastric cancer cell viability,in-hibited their proliferation and migration,and induced apoptosis and autophagy(P＜0.05),and the combination of the two had a synergistic effect(P＜0.05).Esomeprazole and AG1478 both reduced the expression of PI3K,AKT and P-AKT proteins in AGS and SGC-7901 cells(P＜0.05),and the combination of the two had a synergistic effect on reducing the expression of P-AKT(P＜0.05).Conclusion:Esomeprazole combined with the EGFR inhibitor AG1478 has inhibitory effects on the proliferation and migra-tion of gastric cancer cells,and they synergistically induce apoptosis and autophagy,the combination of which may be a potential individualized treatment strategy.
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