Analysis of subgingival plaque microbiota community structure in moderate to severe periodontitis and cerebral infarction patients based on 16S rDNA sequencing
Objective:To compare the microbial community structure and diversity of subgingival plaque in patients with mod-erate to severe periodontitis and cerebral infarction by 16S rDNA sequencing,and to explore the relationship between moderate to severe periodontitis and cerebral infarction.Methods:According to the inclusion and exclusion criteria,subgingival plaque was col-lected from 10 patients with moderate to severe periodontitis(the PD group),10 patients with moderate to severe periodontitis and cerebral infarction(the CI-PD group)and 10 cases of healthy population(the CK group).The 16S rDNA V3-V4 region was sequenced by Illumnia NovaSeq 6000 sequencing platform,and bioinformatics analysis was analyzed by Qiime,R,mothur,SPSS and other softwares.Results:The structure of subgingival microflora in the PD and CI-PD groups was similar,but the diversity of subgingival flora in the two groups was significantly different from that in the healthy group(P<0.05).At the level of phylum and genus,the most species in the PD and CI-PD groups were Bacteroidetes and Fusobacterium,and the most species in CK group were Actinobacteria and Fusobacterium.The numbers of Porphyromonas,Prevotella,Treponema,Filifactor and TG5 in micro-biota community of subgingival plaque in the PD and CI-PD groups were higher than those in the CK group(P<0.05),while the numbers of Neisseria,Lautropia,Capnocytophaga,Rothia,Haemophilus and Cardiobacterium in microbiota community of sub-gingival plaque in the CI-PD group were significantly lower than those in the PD group(P<0.05).Conclusion:There are differ-ences in the microbial community structure of subgingival plaque among the CK,PD and CI-PD groups.It is suggested that the in-crease of Porphyromonas,Prevotella,Treponema,Filifactor and TG5 in microbiota community of subgingival plaque of patients with moderate and severe periodontitis with cerebral infarction and the decrease of Neisseria,Lautropia,Capnocytophaga,Rothia,Haemophilus and Cardiobacterium may be related to cerebral infarction.
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