ATP1B3 expression in gliomas and its effect on cell proliferation and migration
Objective:To investigate the expression of ATP1B3 in glioma and its effect on the proliferation and migration of U87MG cells.Methods:Online databases CGGA and TCGA were used to analysis the differential expression of ATP1B3 in different grades of glioma cells and its relationship with patient survival and prognosis.siRNA interference was used to transiently knock down the expression level of ATP1B3 in the glioma cell line U87MG,and the efficiency of the knockdown was detected by QRT-PCR and western blot methods;Changes in proliferation and migration ability of glioma cells after knockdown of ATP1B3 were detected by CCK-8 and transwell assay;changes in expression of P-MTOR and MTOR after knockdown of ATP1B3 were detected by western blot assay.Results:Database analysis showed that the expression of ATP1B3 was positively correlated with the malignant degree of malignant glioma and negatively correlated with the prognostic survival of patients.The mRNA and pro-tein expression levels of ATP1B3 were significantly reduced after knockdown,and the proliferation and migration ability of cells in the knockdown group was significantly lower than that in the control group(P<0.01);knockdown of ATP1B3 affected the ex-pression level of P-MTOR,and the P-MTOR/MTOR ratio was reduced.Conclusion:High expression of ATP1B3 was associat-ed with the malignancy degree of glioma and unfavorable to the survival prognosis of patients.In glioma cells,ATP1B3 can regu-late the MTOR cell proliferation and growth signalling pathway,and knockdown of ATP1B3 gene can effectively inhibit the prolif-eration and migration of glioma cells,therefore,ATP1B3 gene may be a potential neural tumor marker and a molecular target for therapy.
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