Effects of FCN3 on proliferation,migration and epithelial-mesenchymal transformation of hepatocellular carcinoma cells
Objective:To investigate the expression of Ficolin-3(FCN3)in hepatocellular carcinoma(HCC)and its effects on cell proliferation,migration and epithelial-mesenchymal transformation(EMT).Methods:Firstly,bioinformatics was used to ana-lyze the expression of FCN3 in HCC and its relationship with the prognosis of patients.Then,the expressions of FCN3 in HCC cells and tissues were further detected by qRT-PCR and immunohistochemistry.Huh7 stable cell lines overexpressing FCN3 were constructed,and the effects of FCN3 on the proliferation,migration and invasion of HCC cells were detected by CCK-8,scratch and Transwell assays.The expression levels of E-cadherin,N-cadherin and Vimentin were detected by Western Blot assay.The effect of FCN3 on HCC proliferation was investigated in vivo by subcutaneous tumor formation in nude mice.Results:Bioinfor-matics analysis showed that the expression level of FCN3 in HCC tissues was significantly lower than that in paracancer tissues,and HCC patients with high expression of FCN3 had longer overall survival time.qRT-PCR showed that mRNA expression levels of FCN3 in HCC cell lines Huh-7,Hep3B and SNU-449 were significantly decreased compared with normal hepatocyte.Immuno-histochemistry showed that the proportion of FCN3 staining positive cells in HCC tissues was significantly lower than that in para-cancer tissues.The Huh7 cell line with the relatively lowest expression of FCN3 was selected for lentivirus stable FCN3 gene,and stable OE-Huh7 and no-load lentivirus stable NC-Huh7 were obtained.Transwell,scratch and CCK8 tests showed that the migra-tion,proliferation and invasion ability were significantly reduced in OE-Huh7 group compared with NC-Huh7 group.Western Blot results showed that the expression levels of Vimentin and N-cadherin protein decreased while the expression levels of E-cadherin protein increased in OE-Huh7 group compared with NC-Huh7 group.Conclusions:The expression of FCN3 is low in HCC,and FCN3 affects the prognosis of patients.Overexpression of FCN3 can inhibit the proliferation,migration and EMT of HCC cells.
万方数据
维普
