M2 macrophages regulate the proliferation,migration and invasion of gallbladder cancer cells by secreting interleukin-10
Objective:To investigate the effect of M2 macrophages on the proliferation,migration and invasion of gallbladder carcinoma cells by regulating the expression of interleukin-10(IL-10).Methods:Human monocyte line THP-1 was induced into M2 macrophages in vitro,and the expression of corresponding markers and IL-10 was detected by RT-qPCR.The expression of IL-10 in cell supernatant was detected by ELISA.GBC cell lines NOZ and GBC-SD were divided into the Control group(serum-free medium)、the M2-CM group(M2 macrophage conditioned medium)、the M2-CM+anti-IL-10 group(M2-CM containing anti-IL-10)and the M2-CM+IgG group(M2-CM containing IL-10 isotype control antibody).The cell proliferation rate of each group was measured by CCK-8 test,the cell migration rate of each group was detected by cell scratch test and the change in cell in-vasive ability of each group was detected by Transwell test;and Western blot assay was used to detect the expression of epithelial-mesenchymal transformation markers(E-cadherin and Vimentin)in each group.Results:M2 macrophages were successfully in-duced in vitro.Compared with uninduced THP-1,IL-10 was highly expressed in M2 macrophages and M2 macrophages superna-tant(P<0.05).M2 macrophages could promote the proliferation,migration and invasion of GBC cells,as well as inhibit the ex-pression of E-cadherin and promote the expression of Vimentin.Blocking IL-10 could inhibit the effect of M2 macrophages(P<0.05).Conclusion:M2 macrophages can promote the proliferation,migration,invasion and epithelial-mesenchymal transforma-tion of GBC cells by secreting IL-10.
Gallbladder carcinomaM2 macrophageInterleukin-10ProliferationMigration and invasion
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