ATP2A1 regulates proliferation and migration in colorectal cancer and serves as a predictor of patient prognosis and immune infiltration
Objective:To investigate the mechanism of endoplasmic reticulum Ca2+-translocating ATPase isoform 1(ATP2A1)involved in influencing the biological behavior and immune infiltration of colorectal cancer(CRC).Methods:Tran-scriptome and immunohistochemistry(IHC)were used to analyze the relationship between ATP2A1 expression in colorectal can-cer tumors and the prognosis of colorectal cancer patients,and GO and KEGG were used to analyze the cellular functions and sig-naling pathways that may be involved in the expression level of ATP2A1.The ESTIMATE algorithm was used to analyze the ef-fect of ATP2A1 expression on the tumor microenvironment and the mutational load of tumors.The CCK8 proliferation assay and flow cell cycle assay were used to detect the mechanism of ATP2A1 involved in colorectal cancer proliferation,and the mechanism of ATP2A1 involved in colorectal cancer migration was analyzed by using scratch wound healing assay,Transwell assay and West-ern blot assay.Results:Colorectal cancer patients were divided into two groups of high and low expression based on the median val-ue of ATP2A1 expression.Kaplan-Meier curves and Cox regression analyses demonstrated that high ATP2A1 expression was a prognostic risk factor for colorectal cancer patients,and the results of the ESTIMATE algorithm demonstrated that ATP2A1 ex-pression decreased the abundance of immune cell infiltration,increased the tumor mutational load(TMB)and tumor cell stem-ness.The results of CCK8 proliferation assay and cell cycle assay showed that knockdown of ATP2A1 expression caused S-phase blockage in rectal cancer cells and thus inhibited cell proliferation.The results of scratch wound healing assay,Transwell and West-ern blot assay showed that ATP2A1 could enhance the migration ability of colorectal cancer cells by promoting epithelial mesen-chymal transition of colorectal cancer cells.Conclusion:ATP2A1 is highly expressed in colorectal cancer,which can promote the proliferation and migration of colorectal cancer cells,and affect the prognosis and immunotherapeutic efficacy of colorectal cancer patients.
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