Methyltransferase METTL3 regulates osteogenic differentiation of canine JBMMSCs by mediating m6A methylation
Objective:To explore the effect of methyltransferase METTL3 on the proliferation,migration and osteogenic dif-ferentiation of jaw bone marrow mesenchymal stem cells(JBMMSCs).Methods:JBMMSCs were cultured in vitro and transfect-ed with lentivirus to construct a cell model of silencing METTL3 gene.After transfection,the mRNA level(P<0.001)and pro-tein level(P<0.01)of JBMMSCs in the siMETTL3 group decreased.The proliferation,migration,alkaline phosphatase activi-ty,calcium deposition,and osteogenesis-related factors osteocalcin(OCN)mRNA and protein level of the two groups of JBMMSCs were detected.Results:Compared with the siNC group,the proliferation activity of the siMETTL3 group decreased after 24,48,72,and 96 h of culture(all P<0.001),and the cell migration ability decreased after 24 h(P<0.05).The activity of alkaline phosphatase in the siMETTL3 group decreased after 7 days of osteogenic induction,and the mineralization deposition af-ter 21 days was less than that in the siNC group.qRT-PCR and Western blot showed that the mRNA level(P<0.001)and protein expression level(P<0.05)of OCN reduced.Conclusion:Silencing METTL3 can inhibit the proliferation,migration and osteo-genic differentiation of JBMMSCs.
METTL3Jaw bone marrow mesenchymal stem cellsOsteogenic differentiationm6A methylation
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维普
