Reduction of high glucose-induced oxidative stress in HK-2 cells by Huaiqihuang through activation of the Nrf2/HO-1 signaling pathway
Objective:This study aims to investigate the antioxidant stress effect of HK-2 cells by Huaiqihuang(HQH)through highglucose-induced HK-2 and its Nrf2/HO-1 pathway.Methods:The cells were randomly divided into five groups:nor-mal group,osmotic control group,high-glucose group,HQH group,and captopril group.The cell viability was detected by CCK-8;the level of ROS was detected by fluorescence probe;the activities of SOD,MDA,and GSH content were tested by as-say kits;the expression of Nrf2 was detected by immunofluorescence staining;the mRNA expression levels of IL-6,IL-1β,TNF-α,and Nrf2/HO-1 were detected by qRT-PCR;the expression of Nrf2/HO-1 pathway was detected by Western blotting.Results:Compared with the normal group,the cell viability of the high-glucose group was significantly decreased(P<0.000 1);the levels of ROS and MDA in the cells were increased(P<0.000 1),and the activities of SOD and GSH were decreased(P<0.000 1);The expression of Nrf2 was decreased.the mRNA expression levels of IL-6,IL-1β,and TNF-α were significantly re-duced(P<0.001);the protein and mRNA expression levels of Nrf2/HO-1 were significantly decreased(P<0.001).Compared with the high-glucose group,the cell viability of the HQH groups were significantly increased(P<0.05);the levels of ROS and MDA in the cells were significantly decreased(P<0.000 1),and the activities of SOD and GSH were increased(P<0.01);The expression of Nrf2 was increased.the mRNA expression levels of TNF-α,IL-6,and IL-1β were significantly decreased(P<0.01);the protein and mRNA expression levels of Nrf2/HO-1 were significantly increased(P<0.01).Conclusion:HQH may al-leviate highglucose-induced oxidative stress in HK-2 cells by activating the Nrf2/HO-1 signaling pathway,thus enhancing the body's antioxidant capacity and protecting the cells from oxidative damage.
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