Study on the difference in efficacy of Shenqi Pill based on raw and ripened Dihuang in treating a mouse model with diabetic kidney disease
Objective:To investigate the differences in efficacy and possible related mechanisms between Shenqi Pill with raw and ripened Dihuang in diabetic kidney disease(DKD)mice.Methods:Eight male db/m mice of SPF grade 6-8 weeks old were se-lected as the normal control group(Control group),and another 24 male db/db mice of SPF grade 6-8 weeks old were selected and randomly grouped according to the measurement of blood glucose as follows:the model control group(Model group),the Shenqi Pill raw Dihuang group(S-L group)and Shenqi Pill ripened Dihuang group(S-R group),with 8 db/db mice in each group.After successful modeling,the S-L group and the S-R group were dosed daily with a decoction of Shenqi Pill containing raw and cooked Dihuang(10.4 g/kg),and the normal group and the model group were dosed daily with an equal amount of saline for 56 d.The effects of these two drugs on the levels of fasting blood glucose,24-h urinary protein(UP),microalbumin(mAIB),urinary creatinine(Ucr),total cholesterol(TC),total triglyceride(TG),low-density lipoprotein(LDL-C),high-density lipopro-tein(HDL-C),creatinine(SCr),blood urea nitrogen(BUN),alanine aminotransferase(ALT),aspartate aminotransferase(AST),and bilirubin(TBIL)were measured HE staining was conductedto observe the pathomorphologic changes of renal tis-sues,and Western blot assay to detect protein content of PI3K and Akt in each group.Results:Compared with the control group C,fasting blood glucose,GSP,mAIB,Scr,UP,Ucr,TC,TG,LDL-C,ALT,AST,and TBIL were increased and HDL-C and Ucr were decreased in mice in the model Mo group,and H&E staining showed that glomerular thylakoid membrane hyperpla-sia,glomerular thylakoid cell increase,thylakoid matrix deposition were obvious,and the expressions of PI3K and Akt proteins were down-regulated.Compared with the model group,both the S-L group and the S-R group showed an improvement in bio-chemical indexes,and H&E staining showed that glomerular pathological injury was improved,and the expression of PI3K and Akt proteins were significantly up-regulated,and the difference was statistically significant(P<0.05);however,when comparing the two groups after treatment,BUN,SCr,UP and AST contents of the S-R group were significantly lower(P<0.05).Conclu-sion:Both raw and ripened Dihuang in Shenqi Pill can improve the renal function injury induced by DKD,while the efficacy of the ripened Dihuang group of Shenqi Pill is more significant in the treatment of DKD mice,and the mechanism may be related to the up-regulation of the expression levels of PI3K and Akt in renal tissues.
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维普
