Effect of Yigan Mingmu granules on histomorphology and expression of VEGF and ICAM-1 in diabetes retinal edema rats
Objective:By observing the effect of Yigan Mingmu Granules on the protein expression levels of vascular endothe-lial growth factor(VEGF)and intercellular cell adhesion molecule-1(ICAM-1)in diabetes rats with retinal edema,the mecha-nism of improving diabetes retinal edema was explored.Methods:48 rats were randomly divided into a normal group(8 rats)and a model group(40 rats).Diabetes retinal edema model was established in the model group;At 4 and 8 weeks after the successful establishment of the diabetes model,fundus fluorescein angiography was performed in the normal group and the model group to compare whether the model was successful.The remaining 38 rats that were successfully modeled were randomly divided into model group(7 rats),positive drug group(7 rats),and low,medium,and high dose groups of Yigan Mingmu Granules,with 8 rats in each group;Add 6 remaining rats from the normal group,totaling 6 groups.The normal group and model group were given physiological saline by gavage;The positive drug group was gavaged with 0.09 g/kg Andomin,while the low,medium,and high dose groups of Yigan Mingmu Tang were gavaged with 2.84 g/kg,5.68 g/kg,and 11.36 g/kg Yigan Mingmu Granules,respec-tively.After 4 weeks of treatment,retinal specimens were collected for HE staining to observe the morphology of retinal tissue,optical coherence tomography(OCT)to observe retinal thickness,Western blot to detect the expression levels of VEGF and ICAM-1 in retinal tissue,and ELISA to detect the expression levels of serum VEGF and ICAM-1 protein.Results:Compared with the model group,there was a significant difference in the retinal structure of the treatment group;The retinal thickness of both the positive drug group and the traditional Chinese medicine group decreased significantly(P<0.01);The serum ICAM-1 and VEGF levels in the positive drug group and the traditional Chinese medicine group were significantly reduced(P<0.01);The level of ICAM-1 in the traditional Chinese medicine group decreased(P<0.05),while the level of VEGF significantly decreased(P<0.01).Compared with the positive drug group,the retinal thickness of the low-dose and medium dose YGMMG groups was significantly reduced(P<0.01);Compared with the positive drug group,YGMMG low-dose group,and high-dose group,the se-rum ICAM-1 and VEGF levels in the medium dose group were significantly reduced(P<0.01);Compared with the positive drug group,the ICAM-1 protein level in the medium dose group was significantly reduced(P<0.01).Compared with the high-dose group of YGMMG,the moderate dose group of YGMMG showed a statistically significant decrease in retinal thickness(P<0.05).Compared with the low-dose group,the expression levels of VEGF and ICAM-1 proteins in the medium dose group de-creased(P<0.05).Conclusion:Yigan Mingmu Granules can effectively reduce the degree of retinal edema in diabetes and im-prove the retinal tissue morphology,which may be related to the down-regulation of ICAM-1 and VEGF expression levels.
万方数据
维普
