RNA-Seq-based exploration of the potential mechanism of action of Qingjie Huagong decoction in intervening intestinal barrier injury in rats with severe acute pancreatitis
Objective:To explore the potential mechanism of action of Qingjie Huagong decoction(QJHGD)in intervening the intestinal barrier damage in rats with severe acute pancreatitis by using high-throughput RNA-Seq.Methods:A SAP model was established by retrograde pancreaticobiliary injection of sodium oxybate,HE was used to observe the histopathological chang-es in the pancreas and ileum of rats in the blank group,model group and traditional Chinese medicine group,and the levels of se-rum amylase,lipase,DAO and endotoxin in rats were detected by ELISA method;the differentially expressed genes were detect-ed in ileal tissues of rats in each group by RNA-seq sequencing,so as to screen the core genes,biological functions and signaling pathways of the QJHGD for intervening in the intestinal barrier damage in rats with severe acute pancreatitis.Results:Compared with the blank group,the pancreas and ileum tissues of rats in the model group showed obvious pathological changes,which were significantly improved by the intervention of QJHGD;the ELISA results suggested that QJHGD could significantly improve the serum amylase,lipase,DAO and endotoxin levels in rats with SAP(P＜0.05).The RNA-Seq results showed that there were 2 623 genes differentiated in the SAP group from the BG group,and 175 genes were significantly down-regulated after QJHGD treatment,mainly Tnip3 and Lpo,while 320 genes were up-regulated,mainly aicda and Tnn,and so on.There were 175 genes whose expression was significantly down-regulated after QJHGD treatment,mainly Tnip3,Lpo,etc.,while 320 genes were up-regulated,mainly aicda,Tnn,etc.,which were involved in the regulation of biological processes such as oxidative stress and intervened in multiple signaling pathways such as TJ,apoptosis,Toll-like receptor,NOD-like receptor,NF-κB and so on,and played a role in the regulation of oxidative stress.Conclusion:QJHGD may inhibit the intestinal barrier injury of severe acute pan-creatitis by regulating key genes such as Tnip3 and aicda,as well as multiple signaling pathways such as TJ,apoptosis,and Toll-like receptor.
国家科技期刊平台
NSTL
万方数据
