Research on how miR-21-5p targets BNC2 to control the malignant biological activity of esophageal cancer
Objective:To investigate the mechanism by which the identity transcription factor(BNC2)of myoblasts targeted by miR-21-5p in controling the proliferation,migration,and invasion of esophageal cancer(ESCA)cells.Methods:Real-time quantitative polymerase chain reaction(qRT-PCR)was used to assess the relative expression levels of miR-21-5P and BNC2 in esophageal cancer tissues.And any differences in expression were statistically analyzed.Following transfection of ECA109 and KYSE30 esophageal cancer cell lines with miR-21-5p mimics and miR-21-5P inhibitor,and a negative control(NC,including mimcs NC and inhibitor NC),respectively,the cell function test CCK8,and Transwell was used to investigate the impact of miR-21-5p downregulation or overexpression on cell migration,invasion,and proliferation.A bioinformatics technique was uti-lized to identify and investigate the target genes of miR-21-5P.Then,the dual luciferase assay,Western blot,and qRT-PCR as-say were employed to verify the negative regulatory relationship between miR-21-5p and the target genes.Result:In ESCA tumor tissues,it was possible to determine that BNC2 had reduced relative expression levels.and the relative expression levels of miR-21-5p were greater in tumor tissues by qRT-PCR detection.The outcomes of the CCK8 and Transwell studies demonstrated that overexpression of miR-21-5P increased cell invasion,migration,and proliferation.Cell invasion,migration,and proliferation were all decreased by knocking down miR-21-5P.Put differently,miR-21-5P functions as an oncogene in esophageal cancers.The bioinformatics technique predicted BNC2 to be the target gene of miR-21-5P.According to the results of the dual luciferase assay,miR-21-5P was targeted to wild-type BNC2 ′-UTR.Furthermore,miR-21-5p mimics were shown to be able to drastically lower the BNC2 gene level by western blot and qRT-PCR.On the other hand,a miR-21-5p inhibitor may dramatically raise BNC2 ex-pression in esophageal cancer cells.These findings suggest a link of targeting between miR-21-5P and BNC2.Conclusion:In ES-CA,miR-21-5p targets BNC2,and its regulatory role may contribute to cancer proliferation,migration,and invasion.
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