The ameliorative effects of Vitis vinifera L.Suosuo polysaccharides on Aβ1-42-induced oxidative damage in HT22 cells
Objective:To investigate the effect of deproteinized polysaccharides from Vitis vinifera L.Suosuo(DVTP)on ox-idative damage induced by Aβ1-42 in Alzheimer's disease cell model.Methods:HT22 cells were divided into the control group,the model group(Aβ1-42),and the Aβ1-42+DVTP(20,40,80 μg/mL)groups.Cell viability was measured using the CCK8 assay.Cell morphology and mitochondrial structure changes were observed under light and transmission electron microscopy.ROS levels and mitochondrial membrane potential were assessed using DCFH-DA and JC-10 fluorescent probes,respectively.RT-qPCR and Western blot were used to analyze the mRNA and protein expression levels of Keap1,Nrf2,HO-1,NQO1,and GCLC.Results:Compared to the control group,the model group exhibited decreased cell viability and increased damage to cell morphology and mi-tochondrial structure.ROS levels were elevated,and mitochondrial membrane potential was reduced.The mRNA and protein ex-pression levels of Keap1 were increased,while those of Nrf2,HO-1,NQO1,and GCLC were decreased.In comparison to the model group,DVTP pretreatment significantly improved cell viability,reduced damage to cell morphology and mitochondrial structure,decreased ROS levels,and increased mitochondrial membrane potential.The mRNA and protein expression levels of Keap1 were reduced,while those of Nrf2,HO-1,NQO1,and GCLC were increased.Conclusion:DVTP can mitigate Aβ1-42-in-duced oxidative damage by regulating the expression levels of antioxidant enzymes,suggesting its potential as a candidate drug for the treatment of Alzheimer's disease.
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