Exploration of the mechanism of curculiginis rhizoma sensitizing cisplatin in non-small cell lung cancer based on network pharmacology and experimental verification
Objective:To explore of the mechanism of Curculiginis Rhizoma,a traditional Chinese medicine,sensitizing lung cancer cells to cisplatin chemotherapy.Methods:Network pharmacology and molecular docking techniques were used to screen the main apoptotic proteins of Curculiginis Rhizoma in non-small cell lung cancer;CCK-8 assay was performed to measure cell pro-liferation rate;Western blot was used to detect the expression levels of cleaved Caspase3 and cleaved Caspase9 proteins in lung cancer cells(A549)after treatment with Curculiginis Rhizoma,cisplatin alone,and in combination;flow cytometry was used to detect the total apoptosis rate of cells;cell scratch and invasion experiments were conducted to assess cell migration and invasion capabilities.Results:At a concentration of 100-1 500 μg/mL,the water extract of Curculiginis Rhizoma showed no significant in-hibitory effect on the proliferation of A549 cells.However,the combined treatment showed a significantly enhanced inhibitory ef-fect on A549 cells compared to cisplatin alone.Compared to the control group,the Curculiginis Rhizoma group and the combined treatment group demonstrated significantly increased expression levels of Caspase3 and Caspase9 proteins,total apoptosis rate,as well as cell migration and invasion inhibition rate.The cisplatin group showed significantly increased expression levels of Caspase3 and Caspase9 proteins,as well as the total apoptosis rate,compared to the control group.Additionally,compared to the cisplatin alone group,the Curculiginis Rhizoma group and the combined Curculiginis Rhizoma and cisplatin group demonstrated significant-ly increased expression of Caspase3 and Caspase9 proteins,total apoptosis rate,and cell migration and invasion inhibition rate.Conclusions:Curculiginis Rhizoma,cisplatin,and the combination of Curculiginis Rhizoma with cisplatin can induce apoptosis in A549 cells.The combination with Curculiginis Rhizoma enhances the induction of apoptosis by cisplatin in A549 cells,and its mechanism is related to the apoptosis-associated proteins Caspase3 and Caspase9.Additionally,the combined treatment of Curcu-liginis Rhizoma followed by cisplatin shows a more pronounced inhibitory effect on the proliferation,migration,and invasion capa-bilities of A549 cells.
Non-small cell lung cancerCurculiginis RhizomaCisplatinApoptosis of cellsCaspase3Caspase9
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