Objective:To explore the metabolic markers of acetaminophen-induced acute liver injury in rats through metabolo-mic analysis,and to evaluate the protective mechanism of wild pineapple fruit water extract on liver injury.Method:Thirty rats were randomly divided into 5 groups:control group,model group,wild pineapple extract high-dose group(500 mg/kg),wild pineapple extract low-dose group(200 mg/kg)and the positive drug group(silibinin,30 mg/kg).The administration was contin-ued for 7 days.One hour after each administration,except for the control group,the other groups were given acetaminophen 2 000 mg/kg via gavage.24 H after the administration,blood samples were collected via the abdominal aorta to determine the levels of liver function indicators(ALT,AST)and to conduct histopathological observations.The biomarkers and metabolic pathways of serum samples were analyzed using 1H-NMR.Results:Wild pineapple extract can significantly reduce the serum ALT and AST levels in rats with acute liver injury,and significantly alleviate the pathological damage of the liver,showing the protective effect on the liver,especially at low doses,and its liver protection effect is more prominent than that at high doses.Wild pineapple ex-tract can regulate the metabolic disorder caused by liver injury and increase the levels of acetone and glutamine.Metabonomics anal-ysis revealed 10 potential biomarkers and 6 metabolic pathways.Conclusion:Wild pineapple extract provides protection against acute liver injury caused by acetaminophen by regulating the metabolism of ketone bodies,sugars and amino acids and butyric acid in rat liver,thus effectively improving liver function and alleviating liver injury.
关键词
代谢组学/野菠萝提取物/肝损伤/核磁共振/生物标记物
Key words
Metabonomics/Wild pineapple extract/Liver injury/NMR(nuclear magnetic resonance)/Biomarker
维普
万方数据