MEBT/MEBO promotes diabetic wound healing by regulating TXNIP/NLRP3/Caspase-1 pathway
Objective:To investigate the effect of moist exposed burn therapy/moist exposed burn ointment(MEBT/MEBO)on the expression of TXNIP/NLRP3/Caspase-1 pathway in diabetic wounds.Methods:A total of 48 male Wistar rats were ran-domly divided into the control group,the model group,the MEBO group and the rb-bFGF group.The acute wound model was es-tablished in the control group,and the diabetic wound model was established in the other groups.Then the MEBO group and the rb-bFGF group were treated with moist burn ointment and recombinant bovine basic fibroblast growth factor,respectively.The control group and the model group were treated with normal saline.The wound healing and the expression of TXNIP,NLRP3,and Caspase-1 in each group were observed by H&E staining,immunofluorescence method,and real-time qPCR on the 3rd,7th,and 14th day.Results:⑴ On the 3rd day of treatment,the wound healing rate of the control group was higher than that of the model group,and the difference was statistically significant(P<0.05).On the 7th and 14th day of treatment,the wound healing rates of the control group,MEBO group,and rb-bFGF group were higher than those of the model group(P<0.05).⑵ On the 3rd,7th,and 14th day of treatment,the expression levels of TXNIP and NLRP3 in the control group,MEBO group,and rb-bF-GF group were lower than those in the model group(P<0.05).On the 3rd,7th and 14th day of treatment,the expression levels of Caspase-1 in the control group and MEBO group were lower than those in the model group(P<0.05).Conclusion:MEBT/MEBO can inhibit the expression of TXNIP/NLRP3/Caspase-1 pathway,reduce the inflammatory response of diabetic wound,and thus promote the healing of diabetic wound.
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