Anti-inflammatory Effect of YuPingFeng Base on Network Pharmacology
OBJECTIVE Predicted the key anti-inflammatory targets and signaling pathways of YuPingFeng base on network pharmacology and verified effects in vivo.METHODS The effective components of YuPingFeng and anti-inflammatory targets were screened by TCMSP,GeneCards,OMIM,TTD and PharmGKB database respective-ly.Five effective components and key targets were identified through topology and STRING protein interaction net-work.The intersection targets were uploaded to DAVID database for GO biological function and KEGG signaling path-way enrichment analysis,and molecular docking verification between the key targets and components was carried out.Pharmacodynamic validation was carried out with the mouse model of ear swelling and axillary cotton granuloma inflammation.HE staining was performed on ear tissue and the mRNA of key targets was detected by RT-qPCR.RESULTS 38 effective components were selected,which acted synergistically on 144 inflammatory tar-gets.Intersection targets were mainly involved in biological processes such as response to lipopolysaccharide and to oxidative stress,and they related to signaling pathways such as cancer,TNF and PI3K-Akt.The key targets had strong binding ability with the effective components based on molecular docking results.Each group of YuPingFeng inhibited cotton granuloma on mice(P<0.01),and improved inflammatory cell infiltration and edema in ear tissue signifi-cantly.Compared with model group,YuPingFeng significantly increased the mRNA expression level of TP53,HSP90AA1,JUN and AKT1,and decreased the mRNA expression level of ESRI(P<0.01).CONCLUSION The anti-inflammatory effect of YuPingFeng through multi-component,multi-target and multi-signaling pathway.