首页|基于网络药理学的玉屏风抗炎作用研究

基于网络药理学的玉屏风抗炎作用研究

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目的 以网络药理学方法预测玉屏风抗炎的关键靶点及作用通路,并通过小鼠体内实验进行验证.方法 利用TCMSP、GeneCards、OMIM、TTD及PharmGKB数据库分别筛选出玉屏风活性成分及炎症靶点,通过网络拓扑学与STRING蛋白互作网络分析出5个关键活性成分与靶点,交集靶点上传DAVID数据库进行GO生物功能分析和KEGG信号通路富集分析,并对核心靶点与成分进行分子对接验证.以小鼠耳廓肿胀、腋下棉球肉芽肿炎症模型进行药效学验证,并取小鼠耳廓组织进行HE染色及实时荧光定量PCR法检测核心靶点mRNA表达水平.结果 筛选得38个有效活性成分,协同作用于144个炎症靶点,主要参与脂多糖反应、细胞氧化应激等生物学过程,涉及癌症、TNF和PI3K-Akt等信号通路.分子对接结果显示核心靶点与关键有效成分之间均有较强的结合能力.体内实验结果发现,玉屏风各组显著抑制小鼠棉球肉芽肿(P<0.01),并明显改善耳廓组织中炎性细胞浸润及水肿;与模型组比较,玉屏风可显著提高TP53、HSP90AA1、JUN、AKT1 mRNA表达,降低ESR1 mRNA的表达(P<0.01).结论 玉屏风可通过多成分、多靶点、多通路发挥抗炎作用.
Anti-inflammatory Effect of YuPingFeng Base on Network Pharmacology
OBJECTIVE Predicted the key anti-inflammatory targets and signaling pathways of YuPingFeng base on network pharmacology and verified effects in vivo.METHODS The effective components of YuPingFeng and anti-inflammatory targets were screened by TCMSP,GeneCards,OMIM,TTD and PharmGKB database respective-ly.Five effective components and key targets were identified through topology and STRING protein interaction net-work.The intersection targets were uploaded to DAVID database for GO biological function and KEGG signaling path-way enrichment analysis,and molecular docking verification between the key targets and components was carried out.Pharmacodynamic validation was carried out with the mouse model of ear swelling and axillary cotton granuloma inflammation.HE staining was performed on ear tissue and the mRNA of key targets was detected by RT-qPCR.RESULTS 38 effective components were selected,which acted synergistically on 144 inflammatory tar-gets.Intersection targets were mainly involved in biological processes such as response to lipopolysaccharide and to oxidative stress,and they related to signaling pathways such as cancer,TNF and PI3K-Akt.The key targets had strong binding ability with the effective components based on molecular docking results.Each group of YuPingFeng inhibited cotton granuloma on mice(P<0.01),and improved inflammatory cell infiltration and edema in ear tissue signifi-cantly.Compared with model group,YuPingFeng significantly increased the mRNA expression level of TP53,HSP90AA1,JUN and AKT1,and decreased the mRNA expression level of ESRI(P<0.01).CONCLUSION The anti-inflammatory effect of YuPingFeng through multi-component,multi-target and multi-signaling pathway.

YuPingFengNetwork pharmacologyMolecular dockingAnti-inflammatory

闭锦颜、覃福礼、丁怡萍、罗之昊、焦杨

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广西医科大学药学院,广西南宁 530021

广西医科大学第一附属医院药学部,广西南宁 530021

广西壮族自治区人民医院心血管内科,广西南宁 530021

玉屏风 网络药理学 分子对接 抗炎作用

广西壮族自治区卫生健康委自筹项目

Z-A20220427

2024

海峡药学
中国药学会福建分会

海峡药学

影响因子:0.643
ISSN:1006-3765
年,卷(期):2024.36(1)
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