Effects of Metformin Combined with Curcumin on Apoptosis of Ovarian Cancer SKOV-3 Cells
OBJECTIVE To investigate the effect of curcumin combined with metformin(Met)on SKOV-3 ap-optosis of ovarian cancer cells.METHODS The cultured ovarian cancer cell line SKOV-3 was divided into control group,curcumin group,metformin group and combination group.The effect on cell proliferation inhibition was detec-ted by CCK-8 assay and apoptosis was detected by Annexin V-FITC/PI staining.RESULTS 48 h after SKOV-3 ad-ministration,CCK-8 results showed that the cell growth was time and concentration dependent in the monotherapy group,and the cell growth was more inhibited in the combination group.Annexin V-FITC/PI staining showed that the higher the concentration,the higher the apoptosis rate,and the combination group was stronger than the monotherapy group in promoting apoptosis.CONCLUSION Metformin and curcumin inhibit the growth of SKOV-3 cells in a concentration-dependent manner.Curcumin and metformin in combination has a better effect on inhibiting cell prolif-eration and promoting apoptosis than alone.