Optimize the Extraction Technology of Bletilla striata Polysaccharide by Response Surface Method
OBJECTIVE To investigate theprocess of water extraction and alcohol precipitation of Bletilla stria-ta crude polysaccharides by response surface method,and to separate and purify Bletilla striata crude polysaccharides extracted under the optimal process.METHODS Response surface analysis was used to optimize the process of wa-ter extraction and alcohol precipitation of white and polysaccharide with the yield of polysaccharide as index.The ex-traction temperature,extraction time,solid-liquid ratio and extraction times were investigated as single factors.The ex-traction temperature(A),extraction time(B)and solid-liquid ratio(C)were selected as three major influencing factors.With the extraction rate of polysaccharide content as response value W,the response surface analysis of three factors and three levels of extraction conditions of polysaccharide was carried out.Subsequently,DEAE-650 column and Sepharose-6B column were used to separate and purify Bletilla striata crude polysaccharides.RESULTS The influencing factors of polysaccharide extraction were as follows:B>A>C,the optimum extraction conditions were:extraction temperature 68 ℃,extraction time 94 min,solid-liquid ratio 1:14.Bletilla striata crude polysaccharides were separated and purified by column chromatography to obtain the neutral polysaccharide component BSP-B.CONCLUSION This method is easy to operate and the extraction rate of polysaccharide content is high,which is suitable for the extraction of polysaccharide.Then the crude polysaccharide was separated and purified by column chromatography to obtain the component BSP-B,which laid the foundation for the subsequent experimental study.
Bletilla striata polysaccharideExtractionResponse surfaceProcess optimizationSeparation and purification