Quality Evaluation of Polyrhachis dives from Different Habitats
OBJECTIVE By comparing the contents of extracts,formic acid and met al elements,the quality differences of 21 batches of Polyrhachis dives from 7 producing areas were systematically studied,and a multivariate quality control system for Polyrhachis dives was established.METHODS According to the Chinese Pharmacopoeia(2020 edition),the content of the extract of Polyrhachis dives was determined.Ethanol derivation-headspace gas chromatography(GC)was used to determine formic acid in Polyrhachis dives.The contents of Mg,Ca,Na,K,Fe,Cu,Zn,Mn,Cr,Co,V and Se were determined by microwave digestion and inductively coupled plasma mass spectrometry(ICP-MS).Multivariate statistical analysis was carried out by SPSS combined with cluster analysis and principal component analysis to evaluate the quality difference of Polyrhachis dives from different habitats.RESULTS GC as-say showed that formic acid had good linear relationship in the range of 0.2 mg·mL-1-10 mg·mL-1(R2=1.000),and the recovery rate was 99％-103％.PQ-MS assay showed good precision(RSD=2.07％-5.09％),good repeatability(RSD=2.58％-7.49％),and good linear relationships(R2=0.996-0.999)in the respective concentration ranges.The cluster analysis results showed that the 21 batches of samples could be divided into three categories:S4-S15、S19、S21;S1-S3、S16-S18;and S20 alone.Principal component analysis showed that principal component 1,principal component 2 and principal component 3 were the main factors affecting the quality evaluation of medicinal materials.The cumulative variance contribution rate of the three principal components was 91.339％,and the principal component comprehensive score of S20 was the highest,while that of S21 was the low-est.CONCLUSION Based on the contents of extractives,formic acid and met al elements,the multivariate statisti-cal analysis combined with cluster analysis and principal component analysis can evaluate the quality of Polyrhachis dives from different habitats,and provide reference for the selection of high-quality Polyrhachis dives.
万方数据
维普
