Determination of Berberine Hydrochloride in ShenKangLing Capsules by HPLC
OBJECTIVE To establish the HPLC method for determinating the content of berberine hydrochlo-ride in ShenKangLing capsules,so as to provide a basis for quality control of ShenKangLing capsules.METHODS HPLC method was adopted.The determination was performed on a Sapphire-C18 column(4.6 ×250 mm,5 µm)with a mobile phase consisted of acetonitrile-0.1%phosphoric acid solution(gradient elution)at a detection wavelength of 265 nm and a flow rate of 1.0 mL·min-1.The sample size was 10 μL.RESULTS The content of berberine hydrochloride from 0.0584 to 0.5840 μg showed a good linear relationship with peak area value(R2=0.9981),RSD for precision and repeatability test was<2.0%(n=6),RSD for stability test(within 18 hours)was 1.11%,average sample recovery was 98.24%,and RSD=1.43%(n=6).CONCLUSION This method is simple,accu-rate,strong exclusivity,good stability and reproducibility,which can provides a reference for the quality control of ShenKangLing capsules.