Simultaneous Determination of Seven Components in XiaoYanNing Cap-sules by HPLC-VWD-ELSD
OBJECTIVE To develop a method of high performance liquid chromatography-variable wavelength detector-evaporative light scattering detection(HPLC-VWD-ELSD)for simultaneous determination of picfeltarraenin IA,isofraxidin,rosmarinic acid,hyperoside,liquiritin,ilexgenin A chlorogenic acids and ilexsaponin A1 in XiaoYan-Ning Capsules.METHODS HPLC assay was performed on a Waters Symmetry-C18 column(250 mm ×4.6 mm,5μm)with a mixture of acetonitrilel and 0.15%aqueous acetic acid as the mobile phase in gradient elution at the flow rate of 1.0 mL·min-1.Detection wavelength for picfeltarraenin IA,isofraxidin,rosmarinic acid,hyperoside,liquiritin was set at 270 nm and 342 nm;ELSD was used for detection ilexgenin A chlorogenic acids and ilexsaponin A1.The temperature of the drift tube was maintained at 85 ℃ and the nebulizing gas flow rate was 3.5 L·min-1.RESULTS Picfeltarraenin IA,isofraxidin,rosmarinic acid,hyperoside,liquiritin,ilexgenin A chlorogenic acids and ilexsaponin A1 showed good linearity in the ranges of 1.764-35.28 μg,0.6903-13.81 μg,0.9524-19.05 μg,0.4067-8.134 µg,0.2136-4.272 µg,0.9182-18.36 μg and 0.7665-15.33 μg(r≥0.9991),the average re-coveries and RSD were 100.01%(0.81%),99.14%(0.84%),99.40%(0.92%),99.34%(1.02%),99.20%(1.04%),98.83%(1.09%),98.99%(1.08%)respectively.The contents of the above mentioned components in four samples were 6.812-6.901 mg·g-1,1.726-1.789 mg·g-1,2.391-2.422 mg·g-1,1.134-1.155 mg· g-1,0.6087-0.6105 mg·g-1,2.306-2.324 mg·g-1 and 1.995-2.016 mg·g-1,respective-ly.CONCLUSION The method is simple and reproducible.It can be used for the determination of the seven com-ponents in XiaoYanNing Capsules,providing a scientific basis for the comprehensive quality control of XiaoYanNing Capsules.
XiaoYanNing CapsulesHPLC-VWD-ELSDPicfeltarraenin IAIsofraxidinRosmarinic acidHyperosideLiquiritinIl-exsaponin A1Ilexgenin A chlorogenic acids