Preparation and in vitro Release of Doxorubicin Hydrochloride Liposome
OBJECTIVE To prepare doxorubicin hydrochloride liposome(DOX-Lip)and investigate its in vitro release behavior.METHODS DOX-Lip was prepared by ammonium sulfate gradient methods.The encapsulation ef-ficiency of doxorubicin were investigated by a single factor of ammonium sulfate pH,oil to water ratio,extrusion pas-ses,diafiltration passes,incubation temperature and duration.Taking encapsulation efficiency as evaluation indica-tors,the orthogonal test was used to optimize the formulation process of DOX-Lip and investigate in vitro release be-havior.RESULTS DOX-Lip which prepared by optimal formulation process was spherical with a uniform size distri-bution.The particle size was 85 nm-87 nm(PDI =0.052-0.078),the Zeta potential was-15 mV--16 mV and the encapsulation efficiency was 96%-97%.DOX-Lip released slowly in vitro and was similar to that of the ref-erence listed drug(RLD).CONCLUSION DOX-Lip prepared by the optimal formulation process is stable and feasible,has obvious sustained-release characteristics and is similar to that of RLD,and provides a research basis for the bioequivalence evaluation of doxorubicin hydrochloride liposome injection.
Doxorubicin hydrochlorideLiposomesOrthogonal testin vitro drug release
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