Screening of Anti-tumor Polysaccharides from Traditional Chinese Medi-cine Based on Multiple Cytokine Secretion
OBJECTIVE Cytokines in tumor microenvironment play important roles in tumor progression.This study aimed to screen anti-tumor polysaccharides of traditional Chinese medicine based on cytokine re-lease.METHODS 20 Chinese herbal polysaccharides were selected and screened by Cytometric Bead Array(CBA)-flow cytometry assay.The in vivo anti-tumor effect of active polysaccharides were tested in melanoma B16F10 bearing mice.RESULTS 17 Chinese herbal polysaccharides could significantly stimulate the secretion of TNF-α from splenocytes.5 polysaccharides could stimulate the secretion of IL-6.Safflower polysaccharides and Sagittaria pol-ysaccharides could also significantly stimulate the secretion of IFN-γ.Furthermore,Safflower polysaccharide could in-hibit the growth of melanoma B16F10 cells in mice.CONCLUSION The assay of multiple cytokines' production could be used as a screening method for cancer immunotherapy from Chinese medicine.Safflower polysaccharide can evoke immune response and has anti-tumor effects.
Cancer immunotherapyCytokinesPolysaccharides of Traditional Chinese MedicineSafflower polysaccharides
NETL
NSTL
万方数据
