Study on the Toxicity and Analgesic Effect of Gelsemium elegans with Dif-ferent Fermentation Days
OBJECTIVE To study the toxicity and analgesic effects of Gelsemium elegans with different fermen-tation days.METHODS The modified Kou's method was used to determine the median lethal dose(LD50)of Gel-semium elegans fermented for 14 and 21 days,and the maximum safe dosage of the two batches of Gelsemium elegans was obtained.The acetic acid writhing method was used to determine the analgesic effect of fermented Gelsemium ele-gans,and the ELISA method was used to detect TNF-α,IL-1β and IL-6 levels in the serum of mice.RESULTS The LD50 of fermented Gelsemium elegans for 14 and 21 days was 10.91 and 15.4 g·kg-1,respectively;The analge-sic rates of the two batches of fermented Gelsemium elegans in the acetic acid writhing model were 74%and 79%,respectively.By comparison with the model group,two batches of fermented Gelsemium elegans reduced the content of serum inflammatory factors significantly,but there was no significant difference between the two bat-ches.CONCLUSION Gelsemium elegans showed reduced toxicity and significant analgesic effects after 14 or 21 days fermentation.
NETL
NSTL
万方数据
