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蒲黄的挥发油成分及其体外抗炎、抗氧化活性研究

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目的 分析蒲黄挥发油的化学成分,评价其体外抗炎和抗氧化活性.方法 采用乙醚回流提取法结合气流吹扫-微注射器萃取技术(GP-MSE)提取蒲黄挥发油,气相色谱-质谱联用仪(GC-MS)对挥发油成分进行分析并鉴定,归一化法计算各成分的相对含量;MTT法检测蒲黄挥发油对RAW264.7 细胞的增殖毒性,通过脂多糖(LPS)诱导RAW264.7 细胞炎症反应,Griess法测定炎症因子一氧化氮(NO)含量,初步评价蒲黄挥发油的体外抗炎作用;通过DPPH自由基清除率和Fe3+还原能力测定评价蒲黄挥发油的体外抗氧化活性.结果 从蒲黄挥发油中分离出67 个化合物,鉴定其中53 个,占挥发油总量的96.89%,其主要化学成分包括:亚油酸、棕榈酸甲酯、棕榈酸、亚油酸甲酯、油酸甲酯、(Z)-9-十四碳烯醛和十八烷酸等;药效评价实验表明,在本实验浓度范围内,蒲黄挥发油能显著抑制LPS诱导的RAW264.7 炎症细胞中NO的分泌,对DPPH自由基无明显的清除作用,对Fe3+亦无明显的还原作用.结论 蒲黄挥发油的成分以脂肪酸及其简单酯为主,蒲黄挥发油具有一定的体外抗炎活性,但不具有显著的体外抗氧化活性.
Chemical Component,Anti-inflammatory and Antioxidant Activity in vitro of Volatile Oil from Pollen Typhae
OBJECTIVE To investigate the chemical components,anti-inflammatory and antioxidant activity of volatile oil from Pollen Typhae.METHODS The volatile oil was extracted by refluxing extraction with ether and the gas purge microinje extraction(GP-MSE).The volatile oil was analyzed by gas chromatograph-mass spectrometer(GC-MS).Area normalization method was used for quantitative analysis of the volatile oil.MTT assay was used to as-sess the cytotoxicity of volatile oil on RAW264.7.Cellular inflammation was induced by LPS treatment,the content of nitric oxide was determined by griess assay to evaluate the anti-inflammatory activity.The antioxidant activity of vola-tile oil was evaluated by scavenging capacities of DPPH free radicals,and reducing capacity of Fe3+.RESULTS Sixty-seven compounds were isolated from volatile oil of Pollen Typhae,and fifty-three compounds of them were iden-tified,which accounts for 96.89%of the total volatile oils.The main components of the volatile oil included linoleic acid,palmitic acid methyl ester,palmitic acid,linoleic acid methyl ester,oleic acid methyl ester,(Z)-9-tetradecenal and octadecanoic acid.Moreover,the content of linoleic acid was the highest(32.01%).Within the concentration range of this study,the result of griess assay showed that the volatile oil reduced the content of nitric oxide in LPS-in-duced RAW264.7 cells,with a dose-dependent manner.The result of in vivo antioxidant study showed that the vola-tile oil had no obvious scavenging capacity of DPPH free radical,as well,had no obvious reducing capacity of Fe3+.CONCLUSION The main component of volatile oil from Pollen Typhae consisting of aliphatic acid and its ester,the volatile oil had certain anti-inflammatory activity,but had not showed obvious antioxidant activity in vitro.

Pollen TyphaeVolatile oilGas chromatograph-mass spectrometeAnti-inflammatoryAntioxidant

王自梁、郑士超、杨晰淼、安仁波

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云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明 650504

延边大学药学院,吉林延吉 133002

蒲黄 挥发油 气相色谱-质谱联用 抗炎 抗氧化

云南省教育厅科研项目民族药资源化学国家民委教育部重点实验室(云南民族大学)开放基金

2023J0614MZY2210

2024

海峡药学
中国药学会福建分会

海峡药学

影响因子:0.643
ISSN:1006-3765
年,卷(期):2024.36(2)
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