Content Determination and Quality Analysis of Five Components in YanYan Tablets
OBJECTIVE To establish an HPLC method for the simultaneous determination of five components paeoniflorin,baicalin,harpagoside,cinnamic acid and veronicone in YanYan tablets,and to investigate the overall quality of YanYan tablets.METHODS The Agilent Zorbax SB-C18 column(4.6 mm×250 mm,5 μm)was used with the mobile phase of acetonitrile-0.1%phosphoric acid solution with Gradient elution.The detection wavelengths were 230 nm(paeoniflorin),280 nm(baicalin,harpagoside,cinnamic acid)and 213 nm(asparagine);The column temperature was30℃;The flow rate was0.8 mL·min-1.The content determination results of five components were statistically analyzed by SPSS 20.0 software.RESULTS The linear range was 6.35-402.74 μg·mL-1 for paeoni-florin,1.89-120.91 g·mL-1 for baicalin,1.22-77.95 μg·mL-1 for hapaoside,1.04-66.39 μg·mL-1 for cinnam-ic acid and 1.25-80.40 μg·mL-1 for aspartate,respectively.The average recoveries(n =9)ranged from 96.61%to 99.91%.Through the determination of the content of 5 components in 50 batches of pharyngitis tablets,it was found that the quality of YanYan tablets was general,and some manufacturers had the problems of lax control of raw materials,use of substandard medicinal materials or insufficient feeding according to the prescription,and the current quality standards of YanYan tablets need to be improved.CONCLUSION The method has good precision,stability and repeatability,and provides a scientific basis for the quality control of YanYan tablets.
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