Simultaneous Determination of Several Constituents in XinRong Granules by HPLC-CAD
OBJECTIVE To establish a HPLC-CAD method for simultaneously determination the contents of Astragaloside IV,Ophiopogonin D,Rehmannioside D,Schizandrin,Paeoniflorin,Cinnamaldehyde in XinRong gran-ules.METHODS The analysis was conducted on the Atlantis PREMIER BEH C18(4.6×250 mm,5 μm),which maintained at 35℃column temperature,methanol(a)-0.1%acetic acid aqueous solution(b)was the mobile phase in a gradient elution mode while the flow rate was 1.0 mL·min-1,the nebulization temperature of the detector was 35℃,and the injection volume was 10 μL.RESULTS The linear ranges of Astragaloside IV,Ophiopogonin D,Rehmannioside D,Schizandrin,Paeoniflorin,Cinnamaldehyde were good.The average recoveries of the six compo-nents were 98.9%,98.4%,98.7%,99.4%,100.1%,101.3%;and the RSD were 0.8%,1.4%,1.4%,0.7%,0.9%,0.6%respectively.CONCLUSION The method can be used for the determination of XinRong granules.
万方数据
维普
