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紫外-可见分光光度法测定舒筋消肿散中双酯型生物碱限量

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目的 建立舒筋消肿散中双酯型生物碱的限量测定方法.方法 应用紫外-可见分光光度法,采用改良盐酸羟胺-高氯酸铁法显色,在520 nm波长处测定乌头碱对照液和供试品溶液的吸光度,计算供试品溶液中双酯型生物碱的含量.结果 乌头碱对照液浓度在23.1～231.0 μg·mL-1范围内线性关系良好,回归方程A =0.00705C +0.000134,r =0.999891.乌头碱平均回收率92.42%,RSD =1.20%(n =9).测得10批舒筋消肿散中双酯型生物碱限量,以乌头碱计分别为:0.131%、0.065%、0.077%、0.117%、0.108%、0.081%、0.097%、0.122%、0.103%、0.092%.结论 建立的测定方法简便、准确、灵敏、重现性良好,适用于舒筋消肿散中双酯型生物碱限量的测定.
Determination of Diester Alkaloids in ShuJin XiaoZhong Powder by Uler-aviolet Visible Spectrophotometry
OBJECTIVE Establish the limit method of diester alkaloids in ShuJin XiaoZhong pow-der.METHODS Determination of absorbance of the aconitine solution and the test solution at 520 nm and calcu-late the content of diester alkaloids in the test solution adoption of ulteraviolet visible spectrophotometry and modified hydroxylamine hydrochloride-iron perchlorate color process.RESULTS There was a good linearity within the limit of aconitine during 23.1-231.0 μg·mL-1.The equation of regression was A = 0.00705C + 0.000134,r = 0.999891.The average recovery of aconitine was 92.42%,RSD =1.20%(n =9).The limits of diester alkaloids in 10 batches of ShuJin XiaoZhong powder were 0.131%,0.065%,0.077%,0.117%,0.108%,0.081%,0.097%,0.122%,0.103%,0.092%according to aconitine respectively.CONCLUSION The established method is sim-ple,accurate,sensitive and reproducible.It is suitable for the determination of diester alkaloids in ShuJin XiaoZhong powder.

ShuJin XiaoZhong powderUleraviolet visible spectrophotometryDiester alkaloids

叶希奇、吴军军、潘丕阑

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福建中医药大学附属宁德中医院,福建宁德 352100

舒筋消肿散 紫外-可见分光光度法 双酯型生物碱限量

2024

海峡药学
中国药学会福建分会

海峡药学

影响因子：0.643
ISSN：1006-3765
年,卷(期)：2024.36(3)
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