Determination the Total Amino Acids in Oyster Shell by Ultraviolet visible Spectrophotometry
OBJECTIVE To establish the method for determination of the total amino acid in oyster shell.METHODS Determination of the total amino acids in oyster shell by ultraviolet visible spectrophotome-try.The chromogenic agents were ninhydrin ethanol solution and phosphate buffer.The detection wavelength was 563 nm.RESULTS It showed a good linear relationship of glycine within the range of 20.3-412μg·mL-1(r = 0.9997).The sample recovery was 98.59%,RSD =2.95%(n =6).The total amino acids in oyster shell come from 6 different manufactures and batches were 1.88 mg·g-1,2.24 mg·g-1,1.76 mg·g-1,2.15 mg·g-1,2.03 mg·g-1 and 2.04 mg·g-1 respectively.CONCLUSION The established method is simple,accuracy,sensitivity and repeatability,which was suitable for determination of the total amino acid in oyster shell.
万方数据
维普
