Study on HPLC-ELSD Fingerprint of Periploca forrestii Schltr.
OBJECTIVE To establish the fingerprint of Periploca forrestii Schltr.for quality evaluation.The quality of medicinal materials was evaluated by cluster analysis and principal component analysis.METHODS High performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD)method was used to es-tablish the fingerprints of 17 different habitats of Periploca forrestii Schltr.The chemical constituents of Periploca for-restii Schltr.were characterized by high performance liquid chromatography-quadrupole-time of flight mass spectrom-eter,and the quality of the medicinal materials was evaluated by fingerprint similarity evaluation system,hierarchical cluster analysis and principal component analysis.RESULTS A total of 14 common peaks were identified in the fingerprint,and 58 compounds were identified by liquid chromatography-mass spectrometry.Except for the similarity of 0.663 and 0.826 between the fingerprints of the two batches of Guangxi origin and the control map,the similarity of the fingerprints of the other 15 batches of origin and the control map was above 0.9.The results of fingerprint simi-larity evaluation,hierarchical cluster analysis and principal component analysis were mutually con-firmed.CONCLUSION The study of the fingerprint of Periploca forrestii Schltr.combined with cluster analysis and principal component analysis provides a reference for the authenticity identification and quality control of this medici-nal material.
Periploca forrestii Schltr.FingerprintCluster analysisPrincipal component analysisQuality control
万方数据
维普
