摘要
目的 研究阿胶和驴骨胶中矿物元素的含量差异,寻找可鉴别阿胶和驴骨胶的特征性指标.方法 采用电感耦合等离子体质谱分析方法,对阿胶、驴骨胶、混合胶中的Ca、P、Mg、Zn、Fe、Cu、Al、Sr元素进行含量测定.采用主成分分析及Mann-Whitney检验对阿胶和驴骨胶中元素含量的差异进行统计分析.结果 通过主成分分析可将阿胶和驴骨胶明显区分;Ca、Cu、Sr、P、Mg、Fe是造成阿胶和驴骨胶差异的主要元素;驴骨胶和混合胶中P元素和Cu元素含量的比值(P/Cu)高于阿胶.结论 P/Cu值可作为鉴别阿胶和驴骨胶的特征性指标,本研究可为阿胶中驴源性杂质的鉴别提供新的思路和方法.
Abstract
OBJECTIVE This study was aimed to investigate the difference of mineral elements in donkey-hide gelatin(DHG)and donkey-bone gelatin(DBG)and found a special marker to identify them.METHODS The contents of eight mineral elements(Ca,P,Mg,Zn,Fe,Cu,Al and Sr)in DHG,DBG and mixed gelatin samples were analyzed by inductively coupled plasma mass spectrometry.Principal component analysis(PCA)and Mann-Whitney test were used to analyze the differences of mineral elements content between two kinds of gelatins.RESULTS Based on the PCA,DHG and DBG could be clearly distinguished.Six elements,including Ca,Cu,Sr,P,Mg and Fe,were the characteristic elements of two gelatins.The ratio of elemental P to elemental Cu content(P/Cu)in DBG and mixed gelatin was higher than DHG.CONCLUSION The P/Cu value can be used to identify the DHG and DBG.This study may provide new ideas and methods for the identification of impurities derived from donkey in DHG.
基金项目
广东省基础与应用基础研究基金(2020A1515010577)
广东省中医药信息化重点实验室项目(2021B1212040007)
维普
万方数据