OBJECTIVE To investigate the effect of JiaWei SiShen Soup on growth and development through the study of its inhibitory effect on oxidative stress injury in zebrafish embryos.METHODS Zebrafish at 7-9 hpf af-ter fertilization were randomly divided into a blank control group,a model group,and three garnished SiShen Tang-treated groups(at concentrations of 1.34 mg·mL-1,0.592 mg·mL-1,and 0.1974 mg·mL-1,respectively),and were exposed to 5 mmol·L-1 acrylamide(AA)solution,and then intervened with JiaWei Sishen Soup to observe and recorded various growth parameters of 24 hpf zebrafish embryos.RESULTS AA exposure caused pericardial e-dema and spinal curvature,resulting in hatching disorders and delayed yolk sac absorption.After the intervention of JiaWei SiShen Soup,the zebrafish embryos were improved with malformations and developed well in all growth pa-rameters.Under the effect of the concentration of 0.592 mg·mL-1 and 1.34 mg·mL-1 of JiaWei SiShen Soup,the 1 body length of the juvenile fish was significantly different from that of the control group(P<0.05 or 0.01),which indicated that the JiaWei SiShen Soup had an inhibitory effect on the oxidative stress damage of zebrafish embryos in-duced by AA.CONCLUSION JiaWei SiShen Soup has a better promotion effect on the growth and development of zebrafish.
关键词
加味四神汤/丙烯酰胺/斑马鱼/氧化应激损伤/生长发育
Key words
JiaWei SiShen Soup/Acrylamide/Zebrafish/Oxidative stress injury/Growth and development
维普
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