OBJECTIVE This study aimed to comprehensively analyze the chemical constituents present in the dried rhizomes of Panax japonicus.METHODS The Ethanol Extracts was separated and purified by column chro-matography and liquid phase preparation and the isolated compounds were identified and their structures were eluci-dated through analysis of their physicochemical properties,nuclear magnetic resonance(NMR)spectroscopy,and mass spectrometry(MS)data.RESULTS A total of eleven saponin compounds were successfully isolated and iden-tified from the dried rhizomes of Panax japonicus.The structures and names of these compounds are as follows:Gin-senoside Re(1),Ginsenoside Rg1(2),Chikusetsusaponin V(3),24-(R)-Majoroside R1(4),Chikusetsusaponin Ⅳa(5),Chikusetsusaponin Ⅳa Methyl Ester(6),Chikusetsusaponin Ⅳ(7),Chikusetsusaponin Ⅳ Methyl Ester(8),Chikusetsusaponin Ⅰ(9),Calenduloside E(10),Chikusetsu-saponin IVa Ethyl Ester(11).CONCLUSION Com-pounds 4,10,and 11 were isolated for the first time from Panax Japonicus.