Analysis of Appropriate Concentration of Micrococcus luteus Suspension in Tetracycline Residue Test Method
OBJECTIVE To determine the appropriate concentration range of Micrococcus luteus suspension in tetracycline residue inspection method.METHODS The growth curve of Micrococcus luteus was investigated with reference to the four appendixes 3408 of the CP 2020 for the inspection method of antibiotic residues(cultivation method)and the"Manual for Microbiological Inspection of Drugs".When the standard concentration of tetracycline was verified to be 0.125 μg·mL-1 by the tube-disk method,the antibacterial properties of Micrococcus luteus CMCC(B)28001 at different concentrations in the growth phase.RESULTS The experiment proved that 0.125 μg·mL-1 tetracycline reference solution could not produce bacteriostatic effect on Micrococcus luteus.CONCLUSION The tetracycline residue inspection method needs to replace the standard strain or adjust the standard concentration.
万方数据
维普
